Study of Glycosaminoglycans Sulphate (GAGs) Recovery Efficiency from the Hydrolysis of Chicken Kneel Cartilage by Alcalase 2.4L

Thi Ngoc Binh Nguyen1, , Thi Thua An Trinh1, Uy Thu Nguyen1, Thi Anh Dao Dong1
1 Trường Đại học Bách khoa, Đại học Quốc gia Thành phố Hồ Chí Minh, Việt Nam

Main Article Content

Abstract

Glycosaminoglycans sulphate (GAGs) belonging to a family of proteoglycans are complex natural polysacchrides in animals. Chondroitin sulfate (CS) is a major class of Glycosaminoglycans sulphate used for treatment of arthritis, especial osteoarthritis. Glycosaminoglycans sulphate can be extracted from cartilage of as cows, pigs, chicken, sharks, etc. The extraction of Glycosaminoglycans sulphate from agricultural waste sources for food supplements is meaningful for Vietnam to substitute the exported material. Our research is aimed to determine the optimal condition of hydrolysis of chicken keel cartilage by Alcalase 2.4 L to obtain Glycosaminoglycans sulphate. The optimal values for the hydrolysis process are pH at 7,0; temperature at $54^{\circ}C$; the ratio of enzyme and substances at 4 %V/Wpro and hydrolysis time of 212 minutes. Meanwhile, Glycosaminoglycans sulphate sulfate content are obtained according to the predictions of the model at 27,78% (dry weight of raw material). Glycosaminoglycans sulphate powder product was found to be Glycosaminoglycans sulphate: 53,61% (dry basis); Chondroitin sulfate determined by HPLC: 37,32% (dry basis).

Article Details

References

[1]. Johanne Martel-Pelletier, Aina Farran, Eulàlia Montell, Josep Vergés and Jean-Pierre Pelletier (2015) Discrepancies in Composition and Biological Effects of Different Formulations of Chondroitin Sulfate. Molecules 20, 4277-4289.
[2]. J.C.F. Kwok et al (2012) Chondroitin sulfate: A key molecule in the brain matrix. The International Journal of Biochemistry & Cell Biology, vol. 44, 582-586.
[3]. Tadahisa Mikami et al (2013) Biosynthesis and function of chondroitin sulfate. Biochimica et Biophysica Acta, vol. 1830, 4719-4733.
[4]. D. Uebelhart et al (1998) Effects of oral chondroitin sulfate on the progression of knee osteoarthritis: a pilot study. Osteoarthritis and cartilage, 39-466
[5]. D. Uebelhart et al (2004) Intermittent treatment of knee osteoarthritis with oral chondroitin sulfate: a one-year, randomized, double-blind, multicenter study versus placebo. Osteoarthritis Cartilage, vol. 12. 269-276.
[6]. M.T. Osterman et al (2007) Current and future anti-TNF therapy for inflammatory bowel disease. Curr Treat Options Gastroenterol, vol. 10, 195-207.
[7]. J.W. Cho et al (2007) Curcumin attenuates the expression of IL-1B, IL-6, and TNF-a as well as cyclin E in TNF-a-treated HaCaT cells; NF-kB and MAPKs as potential upstream targets. International journal of molecular medicine, vol. 19, 469-474.
[8]. C. Jomphe et al (2007) Chondroitin sulfate inhibits the nuclear translocation of nuclear factor-kappaB in interleukin-1beta stimulated chondrocytes. Basic Clin Pharmacol Toxicol, vol. 102, 59-65.
[9]. V.H. Bắc et al (2010) Nghiên cứu tách chiết chondroitin sulfate từ xương sụn cá đuối (dasyatis kuhlii) và cả nhám (carcharhinus sorrah) bằng công nghệ sinh học. Tạp chí viện nghiên cứu hải sản, vol. 16,26-30.
[10]. W.R. Farndale, D.J. Buttle, A.J. Barrett (1986) Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochimca Biophysica Acta 883, 173-177.
[11]. S.C. Shin et al (2006) Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage. Asian-Australasian Journal of Animal Sciences, vol. 19, 601-604.
[12]. C.Y. Cheng et al, "Optimization of microwave-assisted extraction of chondroitin sulfate from tilapia byproduct by response surface methodology," Consumer Electronics, Communications and Networks (CECNet), 2011 International Conference on, pp. 1462-1465, 2011.
[13]. C. Kun-Nan and C. Ming-Ju, "Statistical Optimization: Response Surface Methodology Optimization in Food Engineering." in Optimization in Food Engineering, Ferruh Erdogdu, Ed. New York, USA: Taylor & Francis Group, 2008, ch. 6, pp. 115-141.
[14]. AOAC, Official Methods of Analysis: The Association of Offiial Analytical Chemists. Washington DC, USA, 1999.