Study and Modeling DNA-Preconcentration Microfluidic Device
Main Article Content
Abstract
In this study, to enhance diagnostic efficiency, we focus on the effect of ion concentration polarization (ICP), an electroosmotic (EO) flow, electrophoretic (EP) velocity, and the selective membrane length upon the DNA preconcentration. The study is conducted using the direct simulation of the ions and DNA transport in the electrokinetic system. The transport process is governed by the system of Poisson-Nernst-Planck-Navier-Stokes nonlinear equations. Obtained results show the preconcentrating DNA ability in microfluidic devices, simultaneously point out the implication of the length of the microchannel and selective membranes on DNA plug position. Rely on these results, we proposed an experiment model to increase the efficiency of the DNA preconcentration.
Keywords
Poisson-Nernst-Planck-Navier-Stokes, electrokinetic, deoxyribonucleic acid preconcentration, ion concentration polarization, electroosmosis, electrophoresis
Article Details
References
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[2] X. Wei, V. Q. Do, S. V. Pham, D. Martins, and Y. A. Song, A Multiwell-Based Detection Platform With Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays, Sci. Rep. 8 1 (2018) 10772.
[3] H. Song, Y. Wang, C. Garson, and Kapil Pant, Concurrent DNA preconcentration and separation in bipolar electrode-based microfluidic device, Anal. Methods 7 4 (2015) 1273-1279.
[4] F. Mavre, R. K. Anand, D. R. Laws, K.-F. Chow, B.-Y. Chang, J. A. Crooks, and R. M. Crooks, Bipolar Electrodes: A Useful Tool for Concentration, Separation, and Detection of Analytes in Microelectrochemical Systems, Anal. Chem. 82 21 (2010) 8766-8774.
[5] M. Jia and T. Kim, Multiphysics simulation of ion concentration polarization induced by a surface-patterned nanoporous membrane in single channel devices, Anal. Chem. 86 20 (2014) 10365-10372.
[6] R. Dhopeswharkar, R. M. Crooks, D. Hlushkou, and U. Tallarek, Transient effects on microchannel electrokinetic filtering with an ion-permselective membrane, Anal. Chem. 80 4 (2008) 1039-1048.
[7] F. Moukaileld, L. Mangani, and M. Darwish, The Finite Volume Method in Computational Fluid Dynamics - An Advanced Introduction with OpenFOAM and Matlab, 817, Springer International Publishing Switzerland 113 2016.
[8] R. J. Hunter, Zeta potential in colloid science, 391, Academic Press Inc., San Diego, third printing 1988.
[9] V.-S. Pham, Z. Li, K. M. Lim, J. K. White, and J. Han, Direct numerical simulation of electroconvective instability and hysteretic current-voltage response of a permselective membrane, Phys. Rev. E, Stat. Phys. Plasmas Fluids Relat. Interdiscip. Top. 86 4 (2012) 046310.
[10] J. E. Dennis and R. B. Schnabel, Numerical Methods for Unconstrained Optimization and Nonlinear Equations, 395, Prentice-Hall Inc., Englewood Cliffs, United States of America 1983.
[11] C. Geuzaine and J.-F. Remacle, Gmsh: A three-dimensional finite element mesh generator with built-in pre- and post-processing facilities, International Journal for Numerical Methods in Engineering 79 11 (2009) 1309-1331.
[12] S. R. Mathur and J. Y. Murthy, A multigrid method for the Poisson – Nernst – Planck equations, Int. J. Heat Mass Transf. 52 17–18 (2009) 4031–4039.
[13] H. Daiguji, P. Yang, and A. Majumdar, Ion Transport in Nanofluidic Channels, Nano Letters 4 1 (2004) 137-142.
[14] J. Kim, S. Sahloul, A. Ozolaliev, V. Q. Do, S. Pham, D. Martins, X. Wei, R. Levicky, and Y.-A. Song, Microfluidic Electrokinetic Preconcentration Chips: Enhancing the detection of nucleic acids and exosomes, IEEE Nanotechnol. Mag. 14 2 (2020) 18-34.