Effect of N:P Ratios of the Wastewater Mixture to Growth of Spirulina and N, P Removal Efficiencies after Cultivation
Main Article Content
Abstract
Spirulina is a blue-green alga, which is well-known as a source of protein-rich biomass and well growth in mass scales. The algal biomass can be used as a human diet supplement, animal feeds, and many other applications. Culturing Spirulina in wastewater can utilize nutrients to produce valuable biomass as well as removes pollutants in wastewater. In this study, Spirulina was cultivated in mixtures of diluted rice wine stillage and piggery wastewater for determining the optimal ratio of N:P, collecting protein-rich algae biomass and helping to reduce pollutants in wastewater. The results showed that N:P ratios of wastewater mixture from 16:1 to 20:1 andadding 3 g/L bicarbonate as carbon source was the best conditions for the growth of Spirulina sp.HH. The highest biomass yield was 0.147 g.L⁻¹.day⁻¹, corresponding to the protein content of biomass reaching to 59.8 %. The best removal efficiency of NH₄⁺, NO₃⁻, and PO₄³⁻ were 97.5%, 98.1% and 86.6%, respectively. The study demonstrates the benefit of the reuse of wastewaters to recover the nutrients for producing value-added product.
Keywords
Spirulina, rice-wine stillage, piggery wastewater, N:P ratio, removal efficiency
Article Details
References
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[2] Amha Belay (2007), Chapter 1: Spirulina (Arthrospira): Production and Quality Assurance, edited by M. E. Gershwin Amha Belay, Spirulina in Human nutrition and Health, Taylor & Francis Group, pp 1-26.
[3] Redfield, A. C. Ketchum, B. H, Richards, F.A. The influence of organisms on the composition of seawater. The sea, In: Hill, M.N (Ed.), (1963), Vol.II, page 26-77.
[4] Richard Geider & Julie La Roche (2002), Redfield revisited: Variability of C:N:P in marine microalgae and its biochemical basis, European Journal of Phycology, 37:1 (2002), 1-17.
[5] Hee Jeong Choi & Seung Mok Lee. Effect of the N:P ratio on productivity and nutrient municipal wastewater. Bioprocess and Biosystems Engineering, 38:4 (2015), 761-766.
[6] Xin Yuan, Amit Kumar, Ashish K. Sahu, Sarina J. Ergas. Impact of ammonia concentration on Spirulina platensis growth in an airlift photobioreactort. Bioresource Technology, 102 (2011), 3234–3239.