Synthesis and Evaluation of the Biological Activity of Hydroxyapatite/Chitosan-based Scaffold
Main Article Content
Abstract
In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200 µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold.
Keywords
scaffold, chitosan, apatite, SBF
Article Details
References
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[14] Koutsopoulos, S., Synthesis and characterization of hydroxyapatite crystals. Journal of Biomedical Materials Research. 62(4)(2002) 600-612.
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[2] Rinaudo, M., Chitin and chitosan: properties and applications. Progress in polymer science. 31(2006) 603-632.
[3] Kumar, M.R., et al., Chitosan chemistry and pharmaceutical perspectives. Chemical reviews. 104 (2004) 6017-6084.
[4] Karp, J.M., M.S. Shoichet, and J.E. Davies, Bone formation on two-dimensional poly (DL-lactide-coglycolide)(PLGA) films and three-dimensional PLGA tissue engineering scaffolds in vitro. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 64 (2003) 388-396.
[5] Mano, J. and R. Reis, Osteochondral defects: present situation and tissue engineering approaches. Journal of tissue engineering and regenerative medicine. 1 (2007) 261-273.
[6] Mikos, A.G. and J.S. Temenoff, Formation of highly porous biodegradable scaffolds for tissue engineering. Electronic Journal of Biotechnology. 3(2)2000 23-24.
[7] Venkatesan, J., et al., Preparation and characterization of carbon nanotube-grafted-chitosan–natural hydroxyapatite composite for bone tissue engineering. Carbohydrate Polymers .83(2) (2011) 569-577.
[8] Yu, C.-C., et al., Electrospun scaffolds composing of alginate, chitosan, collagen and hydroxyapatite for applying in bone tissue engineering. Materials Letters. 93 (2013) 133-136.
[9] Vượng, B.X., Tổng hợp và đặc trưng vật liệu composite hydroxyapatite/chitosan ứng dụng trong kỹ thuật y sinh. Tạp chí Khoa học ĐHQGHN: Khoa học Tự nhiên và Công nghệ. Tập 34(Số 1) (2018) 9-15.
[10] Nga, N.K., T.T. Hoai, and P.H. Viet, Biomimetic scaffolds based on hydroxyapatite nanorod/poly (D, L) lactic acid with their corresponding apatiteforming capability and biocompatibility for bonetissue engineering. Colloids and Surfaces B: Biointerfaces .128 (2015) 506-514.
[11] Nga, N.K., et al., Surfactant-assisted size control of hydroxyapatite nanorods for bone tissue engineering. Colloids and Surfaces B: Biointerfaces. 116 (2014) 666-673.
[12] Kothapalli, C.R., M.T. Shaw, and M. Wei, Biodegradable HA-PLA 3-D porous scaffolds: effect of nano-sized filler content on scaffold properties. Acta biomaterialia. 1(6) (2005) 653-662.
[13] Kokubo, T. and H. Takadama, How useful is SBF in predicting in vivo bone bioactivity? Biomaterials, (2006) 2907-2915.
[14] Koutsopoulos, S., Synthesis and characterization of hydroxyapatite crystals. Journal of Biomedical Materials Research. 62(4)(2002) 600-612.
[15] Hoai, T.T. and N.K. Nga, Effect of pore architecture on osteoblast adhesion and proliferation on hydroxyapatite/poly (D, L) lactic acid-based bone scaffolds. Journal of the Iranian Chemical Society 15 (2018) 1663-1671.