Extraction and characterization of galactan from seaweed Gracilaria verrucosa of Vietnam
Main Article Content
Abstract
In this study, we extracted galactan from the seaweed Gracilaria verrucosa of Vietnam and investigated its structure and properties. The seaweed was immersed in water in the different temperatures to obtain the solution of galactan. Thereafter, the solution was coagulated and purified to obtain galactan as a powder. Kjeldahl method, infrared spectroscopy, viscosity determination and differential scanning calorimetry (DSC) were used to characterize structure and properties of the resulting material. It was found that the obtained galactan had agar structure without sulfate as substitute group. The proteins content, the intrinsic viscosity and the thermal resistance of the galactan extracted in water at 40 °C were higher than those of the galactan extracted in water at 100 °C.
Keywords
Gracilaria verrucosa, galactan, structural characterization, intrinsic viscosity, DSC
Article Details
References
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[3] E. Murano, Chemical structure and quality of agars from Gracilaria, J. Appl. Phyco. 7 (1995) 245-254.
[4] R. Iyer, O. De Clerck, J.J. Bolton, V.E. Coyne, Morphological and taxonomic studies of Gracilaria and Gracilariopsis species (Gracilariales, Rhodophyta) from South Africa, S. Afr. J. Bot. 70 (2004) 521-539.
[5] M. Lahaye, Chemistry and physico-chemistry of phycocolloids, Cah. Biol. Mar. 42 (2001) 137-157.
[6] M. Lahaye, C. Rochas, Chemical structure and physico-chemical properties of agar, Hydrobiologia 221 (1991) 137-148.
[7] A.I. Usov, Structural analysis of red seaweed galactans of agar and carrageenan groups, Food Hydrocolloids 12 (1998) 301-308.
[8] C.A. Stortz, A.S. Cerezo, Novel findings in carrageenans, agaroids and “hybrid” red seaweed galactans, Curr. Top. Phytochem. 4 (2000) 121-134.
[9] D.H. Dang, M.H. Hoang, N.S. Pham, Seaweeds from Vietnam used for functional food, medicine and biofertilizer. J. Appl. Phycol. 19 (2007) 817-826.
[10] B.W.S. Souza, M.A. Cerqueira, A. I. Bourbon, A.C. Pinheiro, J.T. Martins, J.A. Teixeira, M.A. Coimbra, A.A. Vicente, Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae, Food Hydrocolloids 27 (2012) 287-292.
[11] E.G. Ordónez, P. Rupérez, FTIR-ATR spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds, Food Hydrocolloids 25 (2011) 1514-1520.
[12] M. Sekkal, J-P Huvenne, P. Legrand, B. Sombret, J-C. Mollet, A. Mouradi-Givernaud, M-C. Verdus, Direct structure identification of polysaccharides from red algae by FTIR microspectrometry I: Localization of agar in Gracilaria verrucosa sections, Mikrochim. Acta 112 (1993) 1-10.
[13] T.T.T. Thanh, H. Yasunaga, R. Takano, H. Urakawa, K. Kajiwara, Molecular characteristics and gelling properties of carrageenan family 2. Tri-sulfated and tetra-sulfated carrageenans, Polym. Bull. 47 (2001)