Antibacterial Activity and Interaction Effects of Litsea Cubeba Fruit Essential Oil and Chitosan Against Food-Borne Bacteria
Main Article Content
Abstract
The aims of this study were to investigate the antibacterial activity and interaction effect of Litsea cubeba fruit essential oil (EO) and chitosan (CTS) against food-borne bacteria. The zone inhibition of EO, CTS and EO-CTS mixture against Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 13061 ranged from 39.0-57.3 mm; 11.7-15.5 mm and 29.7-39.3 mm, respectively. The minimum inhibitory concentration of EO and CTS were 5.53 mg/mL and from 600-800 ppm, respectively. The synergistic effects were found when EO and CTS were used in combination with fractional inhibitory concentration values ranging from 0.62-0.67. Indeed, the concentration of EO and CTS were decreased 6-8 and 2 times in combination comparing with individual treatment, respectively. The obtained results demonstrated the potential application of EO-CTS mixture in food preservation.
Keywords
chitosan, antibacterial activity, Litsea cubea, interaction effect
Article Details
References
[1] P. T. Huyen, Medicinal plant resources in Vietnam, in EBSEA 2014, Exploring Biodiversity for Sustainable Development in South East Asia, 2014, Ha Noi, Vietnam.
[2] Y. Chen, Y. Wang, X. Han, L. Si, Q. Wu, L. Lin, Biology and chemistry of Litsea cubeba, a promising industrial tree in China. Journal of Essential Oil Research, 25(2) (2013) 103-111. https://doi.org/10.1080/10412905.2012.751559
[3] H. Wang and Y. Liu, Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chemistry & Biodiversity, 7(1) (2010) 229-235. https://doi.org/10.1002/cbdv.200800349
[4] H. V. Nguyen, J. C. Meile, M. Lebrun, D. Caruso, S. Chu‐Ky, S. Sarter, Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity. Letters in Applied Microbiology, 66(3) (2018) 207-214. https://doi.org/10.1111/lam.12837
[5] J. R. Calo, P. G. Crandall, C. A. O'Bryan, S. C. Ricke, Essential oils as antimicrobials in food systems - A review. Food Control, 54 (2015) 111-119. https://doi.org/10.1016/j.foodcont.2014.12.040
[6] P. Tongnuanchan and S. Benjakul, Essential oils: extraction, bioactivities, and their uses for food preservation. Journal of Food Science, 79(7) (2014) R1231-R1249. https://doi.org/10.1111/1750-3841.12492
[7] C. F. Sharafati, M. Taghizadeh, M. Rafieian‐kopaei, C. R. Sharafati, Effect of chitosan incorporated with cumin and eucalyptus essential oils as antimicrobial agents on fresh chicken meat, Journal of Food Processing and Preservation, 40(3) (2016) 396-404. https://doi.org/10.1111/jfpp.12616
[8] J. Bonilla, M. Vargas, L. Atarés, A. Chiralt, Effect of chitosan essential oil films on the storage-keeping quality of pork meat products. Food and Bioprocess Technology, 7(8) (2014) 2443-2450. https://doi.org/10.1007/s11947-014-1329-3
[9] H. Wang, and Y. Liu, Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chemistry & Biodiversity, 7(1) (2010) 229-235. https://doi.org/10.1002/cbdv.200800349
[10] J. Gutierrez, C. Barry-Ryan and P. Bourke, Antimicrobial activity of plant essential oils using food model media: efficacy, synergistic potential and interactions with food components, Food Microbiology, 26(2) (2009) 142-150. https://doi.org/10.1016/j.fm.2008.10.008
[11] J. E. Patterson, Mc. E. Leticia , P. W. Nathan, In vitro activity of essential oils against gram-positive and gram-negative clinical isolates, including carbapenem-resistant enterobacteriaceae, Open Forum Infectious Diseases, 6(12) (2019) https://doi.org/10.1093/ofid/ofz502
[12] M. Islam, S. M. Masum, K. R. Mahbub, and M. Haque, Antibacterial activity of crab-chitosan against Staphylococcus aureus and Escherichia coli, Journal of Advanced Scientific Research, 2(4) (2011) 63-66.
[13] S. Zivanovic, S. Chi, A.F. Draughon, Antimicrobial activity of chitosan films enriched with essential oils, Journal of Food Science, 70(1) (2005) M45-M51. https://doi.org/10.1111/j.1365-2621.2005.tb09045.x
[14] S. A. Hammid and F. Ahmad, Chemotype of Litsea cubeba essential oil and its bioactivity, Natural Product Communications, 10(7) (2015) https://doi.org/10.1177/1934578X1501000741
[15] S.O. Voundi, M. Nyegue, I. Lazar, D. Raducanu, F.F. Ndoye, M. Stamate, F.X. Etoa, Effect of essential oils on germination and growth of some pathogenic and spoilage spore-forming bacteria, Foodborne Pathogens and Disease, 12(6) (2015), 551-559. https://doi.org/10.1089/fpd.2014.1892
[16] Li, W. R., Shi, Q. S., Liang, Q., Xie, X. B., Huang, X. M., Chen, Y. B., Antibacterial activity and kinetics of Litsea cubeba oil on Escherichia coli, PLoS One, 9(11) (2014) e110983. https://doi.org/10.1371/journal.pone.0110983
[17] M. E. Badawy and E. I. Rabea, A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection, International Journal of Carbohydrate Chemistry, (2011) 1-29. https://doi.org/10.1155/2011/460381
[18] E. Pereira dos Santos, P. H. M. Nicácio, F. Coêlho Barbosa, H. Nunes da Silva, A. L. S. Andrade, M. V. Lia Fook, I. Farias Leite, Chitosan/essential oils formulations for potential use as wound dressing: physical and antimicrobial properties, Materials, 12(14) (2019) 2223. https://doi.org/10.3390/ma12142223
[2] Y. Chen, Y. Wang, X. Han, L. Si, Q. Wu, L. Lin, Biology and chemistry of Litsea cubeba, a promising industrial tree in China. Journal of Essential Oil Research, 25(2) (2013) 103-111. https://doi.org/10.1080/10412905.2012.751559
[3] H. Wang and Y. Liu, Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chemistry & Biodiversity, 7(1) (2010) 229-235. https://doi.org/10.1002/cbdv.200800349
[4] H. V. Nguyen, J. C. Meile, M. Lebrun, D. Caruso, S. Chu‐Ky, S. Sarter, Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity. Letters in Applied Microbiology, 66(3) (2018) 207-214. https://doi.org/10.1111/lam.12837
[5] J. R. Calo, P. G. Crandall, C. A. O'Bryan, S. C. Ricke, Essential oils as antimicrobials in food systems - A review. Food Control, 54 (2015) 111-119. https://doi.org/10.1016/j.foodcont.2014.12.040
[6] P. Tongnuanchan and S. Benjakul, Essential oils: extraction, bioactivities, and their uses for food preservation. Journal of Food Science, 79(7) (2014) R1231-R1249. https://doi.org/10.1111/1750-3841.12492
[7] C. F. Sharafati, M. Taghizadeh, M. Rafieian‐kopaei, C. R. Sharafati, Effect of chitosan incorporated with cumin and eucalyptus essential oils as antimicrobial agents on fresh chicken meat, Journal of Food Processing and Preservation, 40(3) (2016) 396-404. https://doi.org/10.1111/jfpp.12616
[8] J. Bonilla, M. Vargas, L. Atarés, A. Chiralt, Effect of chitosan essential oil films on the storage-keeping quality of pork meat products. Food and Bioprocess Technology, 7(8) (2014) 2443-2450. https://doi.org/10.1007/s11947-014-1329-3
[9] H. Wang, and Y. Liu, Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chemistry & Biodiversity, 7(1) (2010) 229-235. https://doi.org/10.1002/cbdv.200800349
[10] J. Gutierrez, C. Barry-Ryan and P. Bourke, Antimicrobial activity of plant essential oils using food model media: efficacy, synergistic potential and interactions with food components, Food Microbiology, 26(2) (2009) 142-150. https://doi.org/10.1016/j.fm.2008.10.008
[11] J. E. Patterson, Mc. E. Leticia , P. W. Nathan, In vitro activity of essential oils against gram-positive and gram-negative clinical isolates, including carbapenem-resistant enterobacteriaceae, Open Forum Infectious Diseases, 6(12) (2019) https://doi.org/10.1093/ofid/ofz502
[12] M. Islam, S. M. Masum, K. R. Mahbub, and M. Haque, Antibacterial activity of crab-chitosan against Staphylococcus aureus and Escherichia coli, Journal of Advanced Scientific Research, 2(4) (2011) 63-66.
[13] S. Zivanovic, S. Chi, A.F. Draughon, Antimicrobial activity of chitosan films enriched with essential oils, Journal of Food Science, 70(1) (2005) M45-M51. https://doi.org/10.1111/j.1365-2621.2005.tb09045.x
[14] S. A. Hammid and F. Ahmad, Chemotype of Litsea cubeba essential oil and its bioactivity, Natural Product Communications, 10(7) (2015) https://doi.org/10.1177/1934578X1501000741
[15] S.O. Voundi, M. Nyegue, I. Lazar, D. Raducanu, F.F. Ndoye, M. Stamate, F.X. Etoa, Effect of essential oils on germination and growth of some pathogenic and spoilage spore-forming bacteria, Foodborne Pathogens and Disease, 12(6) (2015), 551-559. https://doi.org/10.1089/fpd.2014.1892
[16] Li, W. R., Shi, Q. S., Liang, Q., Xie, X. B., Huang, X. M., Chen, Y. B., Antibacterial activity and kinetics of Litsea cubeba oil on Escherichia coli, PLoS One, 9(11) (2014) e110983. https://doi.org/10.1371/journal.pone.0110983
[17] M. E. Badawy and E. I. Rabea, A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection, International Journal of Carbohydrate Chemistry, (2011) 1-29. https://doi.org/10.1155/2011/460381
[18] E. Pereira dos Santos, P. H. M. Nicácio, F. Coêlho Barbosa, H. Nunes da Silva, A. L. S. Andrade, M. V. Lia Fook, I. Farias Leite, Chitosan/essential oils formulations for potential use as wound dressing: physical and antimicrobial properties, Materials, 12(14) (2019) 2223. https://doi.org/10.3390/ma12142223