Antimicrobial Interaction between Endophytic Actinobacteria Associated with Listea Cubeba and Listea Cubeba Essential Oils Against Food Borne Bacteria
Main Article Content
Abstract
The purpose of the study was to investigate the antimicrobial activity of endophytic actinomycetes isolated from Listea cubeba (L. cubeba) and its essential oil on two food-borne pathogenic bacteria Gram-positive Escherichia coli ATCC 25922 (E. coli) and Gram-positive Staphylococcus aureus ATCC 25923 (S. aureus) individual and in combination. The results indicated that 3 among 7 endophytic actinomycetes (MPT44, MPT42, MPT 62) showed antibacterial activity against tested strains with the antibacterial diameter ranging from 17 – 44 mm. Using the broth micro-dilution assay, minimum inhibitory concentration (MIC) of endophytic actinomycetes bacteria were from 6.25 to 25 µl/ml. From the indices of fractional inhibitory concentration (FIC) of the combination of L. cubeba essential oil and its endophytic actinomycetes, the synergistic effects were found against E. coli, S. aureus. The combination of L. cubeba essential oil and its endophytic actinomycetes broth culture enhances the antimicrobial efficacy gainst foodborne pathogens 3 to 8 times comparing with individual treatment. In addition, the combination use of essential oils and secondary metabolites from endophytic bacteria should be useful way to enhance food security.
Keywords
Endophytic bacteria, Litsea cubeba, antimicrobial activity, interaction effect, synergy effect
Article Details
References
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[7] P. T. Lacava and C. P. de Sousa, Role of Endophytic Actinomycetes in Crop Protection: Plant Growth Promotion and Biological Control. Plant Growth Promoting Actinobacteria, Springer (2016) 147-160.
[8] F. M. Nongkhlaw and S. R. Joshi, Investigation on the bioactivity of culturable endophytic and epiphytic bacteria associated with ethnomedicinal plants, J. Infect. Developing Countries 9(09) (2015) 954-961.
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[10] S. van Vuuren & A. Viljoen, Plant-based antimicrobial studies-methods and approaches to study the interaction between natural products, Planta Med 77(11) (2011) 1168-1182.
[11] V. T. H. Nguyễn, Đ. T. M. Linh, C. K. Sơn, T. V. Trang and P. Q. Tiến, Phân loại, đặc tính sinh học của Streptomyces griseorubens LCQ8 nội sinh phân lập trên cây Quế lai tại Lai Châu, Kỷ yếu Hội nghị Khoa học Quốc gia lần thứ hai về Nghiên cứu và giảng dạy Sinh học ở Việt Nam, Đà Nẵng 20/05/2016 (2016).
[12] J. Gutierrez, C. Barry-Ryan, and P. Bourke, Antimicrobial activity of plant essential oils using food model media: Efficacy, synergistic potential and interactions with food components, Food Microbiol. 26(2) (2009) 142-150.
[13] A. K. Passari, V. K. Mishra, R. Saikia, V. K. Gupta and B. P. Singh, Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential, Front. Microbiol. 6 (2015) 273-279.
[14] O. Yesil-Celiktas, F. Vardar-Sukan, S. Chandra, H. LATA and A. Varma, Biotechnology for Medicinal Plants: Micropropagation and Improvement, Springer (2013)
[15] J. Li, G. Z. Zhao, H. H. Chen, H. B. Wang, S. Qin, W. Y. Zhu, L. H. Xu, C. L. Jiang and W. J. Li, Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest, Lett. Appl. Microbiol. 47(6) (2008) 574-580.
[16] M. Sadiki, M. Balouiri, H. Barkai, I. H. Maataoui, S. I. Koraichi and S. Elabed, Synergistic antibacterial effect of Myrtus communis and Thymus vulgaris essential oils fractional inhibitory concentration index, Int. J.Pharm. Pharmaceuti. Sci. 6 (6) (2014) 121-124.
[17] N. H. Van, V. T. H. Nguyen, V. T. Trang, P. Q. Tien, K. T. Nhan, T. S. Samira and C. K. Son, Antimicrobial activities and interaction effects of Vietnamese Litsea Cubeba (Lour.) pers essential oil and endophytic antinobacteria, J. Sci Technol. 54 (3A) (2016) 234-241.
[18] W. Li, Q. Shi, Q. Liang, X. Xie, X. Huang, Y. Chen, Antimicrobial activity and kinetics of Litsea cubeba oil on Eschirichia coli. Plos one 9 (11) (2014) 1-6.
[19] F. Nazzaro, F. Fratianni, L. D. Martino, R. Coppola, and V. D. Feo, Effect of essential oils on pathogenic bacteria, Pharmaceuticals 6 (2013) 1451-1474