Study on Isolation of Mangiferin from Mangifera Indica L. Leaves
Main Article Content
Abstract
Mango (Mangifera indica L.) belongs to the family of anacardiaceae, which is a popular tropical plant distributed in Vietnam. Mangiferin (1) was isolated from the leaves of Mangifera indica L. by reflux extraction and ultrasound-assisted extraction methods. Chemical structure of compound 1 was identified by means of spectroscopic methods including electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectroscopy (NMR). Compound 1 showed DPPH scavenging activity with SC₅₀ value of 15.94 µg/ml and moderate cytotoxic activity on four human cancer cell lines including mouth epidermal carcinoma (KB), liver hepatocellular carcinoma (Hep-G2), lung carcinoma (LU-1) and breast cancer (MCF7) with IC₅₀ values of 11.95, 13.75, 15.65 and 16,84 µg/ml, respectively.
Keywords
Mangifera indica, Anacardiaceae, mangiferin, xanthone, DPPH, antioxidant
Article Details
References
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[14] Acosta J., Sevilla I., Salomón S., Nuevas L., Romero A., Amaro D., Determination of mangiferin solubility in solvents used in the biopharmaceutical industry, Journal Pharmacy & Pharmacognosy Research, 4 (2016) 49-53.
[15] S. Faizi, S. Zikr-Ur-Rehman, M. Ali, A. Naz, Temperature and solvent dependent NMR studies on mangiferin and complete NMR spectral assignments of its acyl and methyl derivatives, Magn. Reson. Chem., 44(9) (2006) 838–844.
[2] R.N. Tharanathan, H.M. Yashoda, T.N. Prabha, Mango (Mangifera indica L.), the king of fruits - A review, Food Reviews International, 22 (2006) 95–123.
[3] A.J.N. Sells, H.T.V. Castro, J. A. Agero, J.G. González, F. Naddeo, F. De Simone, L. Rastrelli, Isolation and quantitative analysis of phenolic constituents free sugars, fatty acids and polyols from mango (Mangifera indica L.) stem bark aqueous decoction used in Cuba as nutritional supplement, J. Agric. Food Chem, 50 (2002) 762–766.
[4] S. Guha, S. Ghosal, U. Chattopadhyay, Antitumor, immunomodulatory and anti-HIV effect of mangiferin, a naturally occurring glucosylxanthone, Chemotherapy, 42(6) (1996) 443–451.
[5] M.S. Zheng, Z.Y.Lu, Antiviral effect of mangiferin and isomangiferin on herpes simplex virus, Chinese Medical Journal, 10(1) (1989) 85-90.
[6] Y.X.M. Zhu, J.X. Song, Z.Z. Huang, Y.M. Wu, M.J. Yu, Antiviral activity of mangiferin against herpes simplex virus type 2 in vitro. Zhongguo YaoLi Xuebao, 14(5) (1993) 452–454.
[7] H. Ichiki, T. Miura, M. Kubo, E. Ishihara, Y. Komatsu, K. Tanigawa, M. Okada, New antidiabetic compounds, mangiferin and its glucoside, Biol. Pharm. Bull., 21(12) (1998)1389–1390.
[8] T. Miura, H.Ichiki,I. Hashimoto, N. Iwamoto, M. Kato, M. Kubo, E. Ishihara, Y. Komatsu, M. Okada, T. Ishida, K. Tanigawa, Antidiabetic activity of a xanthone compound, mangiferin, Phytomedicine, 8(2) (2001) 85-87.
[9] P.B. Pal, K. Sinha, P.C.Sil, Mangiferin, a natural xanthone, protects murine liver in Pb(ii) induced hepatic damage and cell death via MAP Kinase, NF-kB and mitochondria dependent pathways; PLoS One, 8 (2013) e56894.
[10] S. Agarwala, K. Mudholkar, R. Bhuwania, B.S. Satish Rao, Mangiferin, a dietary xanthone protects against mercury-induced toxicity in HepG2 cells, Environmental Toxicology, 27 (2012) 117–127.
[11] Trần Hữu Dũng, Nghiên cứu khả năng kích ứng của thuốc nhỏ mắt hỗn dịch mangiferin trên mắt thỏ, Tạp chí Dược học, 53 (2013) 44-49.
[12] S. Gorinstein, O. Martin-Bellos, E. Katrich, A. Lojek, M. Ciz, N. Gligelmo-Miguel, R. Haruenkit, Y.S. Park, Jung, S. Trakhtenberg, Comparison of the contents of the main biochemical compounds and the antioxidant activity of some Spanish olive oils as determined by four different radical scavenging tests, J. Nutr. Biochem. 14 (2003) 154-159.
[13] T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65 (1983) 55–63.
[14] Acosta J., Sevilla I., Salomón S., Nuevas L., Romero A., Amaro D., Determination of mangiferin solubility in solvents used in the biopharmaceutical industry, Journal Pharmacy & Pharmacognosy Research, 4 (2016) 49-53.
[15] S. Faizi, S. Zikr-Ur-Rehman, M. Ali, A. Naz, Temperature and solvent dependent NMR studies on mangiferin and complete NMR spectral assignments of its acyl and methyl derivatives, Magn. Reson. Chem., 44(9) (2006) 838–844.