Direct Glucose Recovery from Cassava bagasse using Granular Starch-Hydrolyzing Enzyme
Main Article Content
Abstract
Biorefinery of biomass which fractionates the biomass to bio-products, is seen as the foundation of the bio-economy. Cassava is main starch source for industry in Vietnam. A long with cassava starch process, cassava bagasse was discharged at a rate of approximately 280 tons of wet bagasse (85% moisture) per every 300 tons of cassava roots processed. With the residue starch estimated up to 50–60%, the bagasse can be seen as a glucose material. By treatment of the bagasse using the granular starch-hydrolyzing enzyme Stargen 002 (DuPont) at 10 GAU/g dry bagasse at 50 °C, pH 3.5-5, the glucose can be totally recovered from residue starch in bagasse in one step process. The highest glucose concentration of the hydrolysate attained of 137 g/L and can be used for fermentation of desired products. Starch-free cellulose received can be used to develop cellulose materials. Those recovered products can help to improve the total value of the cassava production chain.
Keywords
cassava bagasse, glucose recovery, Stargen 002
Article Details
References
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[15] Y. Bai, X. Luo, L. Chang; Optimization of enzymatic hydrolysis and fermentation for bioethanol production from cassava waste; Bioresource Technol. 101(6) (2010) 2077-2081.
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[19] M. Zhang, Y. Zhang, X. Wang; Production of fermentable sugars from cassava bagasse by combined pretreatment and enzymatic hydrolysis; Carbohydr. Polym. 112 (2014) 488-494.
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[3] L.R. Souto, M. Caliari, M.S. Soares Junior, F.A. Fiorda, M.C. Garcia; Utilization of residue from cassava starch processing for production of fermentable sugar by enzymatic hydrolysis; Food Science and Technology 37 (2017) 19-24.
[4] S. Chotinerean, C. Pradtstuwana, P. Sirirtreesas, S. Tantriani; Reducing sugar production from cassava pulp using enzymes and ultra-filtration I: Enzymatic hydrodioloration; J. Sci. Res. Chula. Univ. 29(2) (2004) 119-128.
[5] C.R. Soccol, L.P.S. Vandenberghe; Overview of applied solid-state fermentation in Brazil; Biochem. Eng. J. 13(2) (2003) 205-218.
[6] V. Okudoh, C. Trois, T.S. Workneh, S. Schmidt; The potential of cassava biomass and applicable technologies for sustainable biogas production in South Africa: A review; Renew; Sust. Energ. Rev. 39 (2014) 1035-1052.
[7] C. Balagopahl, R.C. Ray, J.T. Sheriff, L. Rajalekshmy; Biotechnology for the value addition of waste and residues from cassava processing industries; in: Processing of the second international sciencietific meeting of the cassava biotechnology network. Bogor, Indonesia, 22-26 Aug 1994. (1994) 690-701.
[8] A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter, D. Templeton, D. Crocker; Determination os structural carbohydrates and lignin in biomass; Technical Report NREL/TP-510-42618. National Renewable Energy Laboratory, 2011.
[9] G.L. Miller; Use of dinitrosalicylic acid reagent for determination of reducing sugar; Anal. Chem. 31(3) (1959) 426-428.
[10] N.A. Edama, A. Sulaiman, S.N.A. Rahim; Enzymatic hydrodiolation of Tapioca processing wastes into biosugars through immobilization technology (Mini Review); Biofuel Res. J. 1(1) (2014) 2-6.
[11] S. Soccol, L.P. Vandenberghe; Production and properties of cassava starch; Bioresource Technol. 94(2) (2004) 153-162.
[12] M. Pandey, C.R. Soccol, D. Nigam; Solid State Fermentation for Production of High Value Nowterals; in: Applied Biochemistry and Biotechnology 176 (2015) 1-10.
[13] P.S. Reddy, H. Gangadhar, Y.V.R.K. Rao; Enzymatic hydrolysis of Cassava bagasse; Int. J. ChemTech Res. 8(3) (2015) 864-872.
[14] A. Pandey, C.R. Soccol, D. Nigam, M.A. Soccol; Biochemical engineering aspects of sorghum-based bioethanol production; Biotechnol. Adv. 23(1) (2005) 89-105.
[15] Y. Bai, X. Luo, L. Chang; Optimization of enzymatic hydrolysis and fermentation for bioethanol production from cassava waste; Bioresource Technol. 101(6) (2010) 2077-2081.
[16] J. Huang, L. Zheng, Y. Liu; Simultaneous saccharification and fermentation of cassava bagasse for succinic acid production; Bioresource Technol. 162 (2014) 374-381.
[17] R. Oleskowicz-Popiel, S.J. Vancov; Enzymatic hydrolysis of pretreated cassava bagasse; J. Sci. Ind. Res. 71 (2012) 245-250.
[18] F. Kuppens, C. Calus, B. Demeester; Pre-treatment methods for cassava bagasse; Chem. Eng. Trans. 50 (2016) 157-162.
[19] M. Zhang, Y. Zhang, X. Wang; Production of fermentable sugars from cassava bagasse by combined pretreatment and enzymatic hydrolysis; Carbohydr. Polym. 112 (2014) 488-494.
[20] L. Asli, B. Zhang, Z. Yin; Enzymatic hydrolysis of cassava bagasse by immobilized enzyme; Biotechnol. Bioeng. 110(11) (2013) 3096-3104.