Development of a Lateral Flow Immunoassay Test Strip for the Rapid Detection of Rubella Virus
Main Article Content
Abstract
This study was designed to construct a lateral flow immunoassay test strip for rapid detection of rubella virus. Rabbit anti-rubella antibody and pig anti-rubella antibody were successfully purified from sera of rabbit and pig infected with rubella virus. The yields of IgG affinity purification were 1.74 mg/ml and 3.23 mg/ml serum, respectively. Suitable conditions for conjugation of antibody and gold nanoparticles (AuNPs) were determined: 0.5 μg rabbit anti-rubella antibody was conjugated with 10 μl of AuNPs at pH 10, 37°C for 90 min. Similarly, suitable conditions for immobilization of pig anti-rubella antibody on the nitrocellulose were 3 μg of antibody, nitrocellulose membrane of Pall Vivid 170, immobilizing buffer of 20 mM sodium borate plus 2% sucrose, at 37°C for 30 min. The complete test strip was successfully constructed for rapid detection of rubella virus.
Keywords
Rubella virus, lateral flow immunoassay, rapid detection
Article Details
References
[1] WHO, Manual for the laboratory diagnosis of measles and rubella virus infection, WHO/IVB/07.01 (2007).
[2] J.M. Best, S.O’Shea, G. Tipples, N. Davies, S.M. Al-Khusaiby, A. Krause, L.M. Hesketh, L. Jin, G. Enders, Interpretation of rubella serology in pregnancy—pitfalls and problems, The British Medical Journal 325 (2002) 147-148.
[3] K.M. Thompson, E.A. Simons, K. Badizadegan, S.E. Reef, L.Z. Cooper, Characterization of the Risks of Adverse Outcomes Following Rubella Infection in Pregnancy, Risk Analysis 36(7) (2016) 1315-1331.
[4] J.S. Wolinsky, Rubella, in Fields’ Virology, B.N. Fields, D.M. Knipe, P.M. Howley, Editors. 1996, Raven Press: New York, pp. 899-929.
[5] C. Vauloup-Fellous, L. Grangeot-Keros, Humoral Immune Response after Primary Rubella Virus Infection and after Vaccination, Clinical Vaccine Immunology 14(5) (2007) 644-647.
[6] CDC, Epidemiology and Prevention of vaccine-preventable diseases, Chapter 20: Rubella, (2015) 325-340.
[7] S.A. de Oliveira, M.M. Siqueira, D.W.G. Brown, P. Litton, L.A.B. Camacho, S.T. Castro, B.J. Cohen, Diagnosis of rubella infection by detecting specific immunoglobulin M antibodies in saliva samples: a clinic-based study in Niterói, RJ, Brazil, Revista da Sociedade Brasileira de Medicina Tropical 33(4) (2000) 335-339.
[8] G. Zhang, J. Guo, X. Wang, Immunochromatographic lateral flow strip test, chapter 12 in A. Rasooly and K.E. Herold (eds), Methods in Molecular Biology: Biosensors and Biodetection, 504, Humana Press.
[9] M.H. Jazayeri, H. Amani, A.A. Pourfatollah, H. Pazoki-Toroudi, B. Sedighmoghaddam, Various methods of gold nanoparticles (GNPs) conjugation to antibodies, Sensing and Bio-Sensing Research 9 (2016) 17-22.
[10] Milipore. Rapid lateral flow test strips – Consideration for product development. Lit. No. TB500EN00EM (2013), Rev. C 12/13.
[2] J.M. Best, S.O’Shea, G. Tipples, N. Davies, S.M. Al-Khusaiby, A. Krause, L.M. Hesketh, L. Jin, G. Enders, Interpretation of rubella serology in pregnancy—pitfalls and problems, The British Medical Journal 325 (2002) 147-148.
[3] K.M. Thompson, E.A. Simons, K. Badizadegan, S.E. Reef, L.Z. Cooper, Characterization of the Risks of Adverse Outcomes Following Rubella Infection in Pregnancy, Risk Analysis 36(7) (2016) 1315-1331.
[4] J.S. Wolinsky, Rubella, in Fields’ Virology, B.N. Fields, D.M. Knipe, P.M. Howley, Editors. 1996, Raven Press: New York, pp. 899-929.
[5] C. Vauloup-Fellous, L. Grangeot-Keros, Humoral Immune Response after Primary Rubella Virus Infection and after Vaccination, Clinical Vaccine Immunology 14(5) (2007) 644-647.
[6] CDC, Epidemiology and Prevention of vaccine-preventable diseases, Chapter 20: Rubella, (2015) 325-340.
[7] S.A. de Oliveira, M.M. Siqueira, D.W.G. Brown, P. Litton, L.A.B. Camacho, S.T. Castro, B.J. Cohen, Diagnosis of rubella infection by detecting specific immunoglobulin M antibodies in saliva samples: a clinic-based study in Niterói, RJ, Brazil, Revista da Sociedade Brasileira de Medicina Tropical 33(4) (2000) 335-339.
[8] G. Zhang, J. Guo, X. Wang, Immunochromatographic lateral flow strip test, chapter 12 in A. Rasooly and K.E. Herold (eds), Methods in Molecular Biology: Biosensors and Biodetection, 504, Humana Press.
[9] M.H. Jazayeri, H. Amani, A.A. Pourfatollah, H. Pazoki-Toroudi, B. Sedighmoghaddam, Various methods of gold nanoparticles (GNPs) conjugation to antibodies, Sensing and Bio-Sensing Research 9 (2016) 17-22.
[10] Milipore. Rapid lateral flow test strips – Consideration for product development. Lit. No. TB500EN00EM (2013), Rev. C 12/13.