Analysis of Pesticides in Solanum macrocarpon Fruit by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry
Main Article Content
Abstract
A UPLC-MS/MS was developed for the analysis of 11 pesticides in Vietnamese cannon eggplant. Pesticides were extracted by QuEChERS and then cleaned up with solid phase extraction. 11 pesticides in the sample solution were separated by reversed phase column and detected by positive ionization mass spectrometry utilizing with multiple reaction monitoring (MRM). Validation of the developed analytical method was performed by linearity range, limit of detection (LOD), limit of quantification (LOQ), repeatability, recovery, etc. LOD and LOQ ranged from 0.03 µg/kg to 0.1 µg/kg and 0.11 µg/kg to 0.33 µg/kg for wet weight samples, respectively. Repeatability and reproducibility of the analytical method were achieved below 12.3% and 19.1%, respectively. Recovery ranged from 75.5% to 86.6%. Matrix effect was investigated and presented. The validated analytical method was then applied to analyze 11 pesticides in five Vietnamese cannon eggplant samples. Experimental results indicated that some pesticides were detected in the samples analyzed. However, the concentration of these compounds was below the Vietnam Ministry of Health and EU standards.
Keywords
MS/MS, matrix effect, pesticides, QuEChERS, UPLC
Article Details
References
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