Analysis of Pesticides in Solanum macrocarpon Fruit by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry
Main Article Content
Abstract
A UPLC-MS/MS was developed for the analysis of 11 pesticides in Vietnamese cannon eggplant. Pesticides were extracted by QuEChERS and then cleaned up with solid phase extraction. 11 pesticides in the sample solution were separated by reversed phase column and detected by positive ionization mass spectrometry utilizing with multiple reaction monitoring (MRM). Validation of the developed analytical method was performed by linearity range, limit of detection (LOD), limit of quantification (LOQ), repeatability, recovery, etc. LOD and LOQ ranged from 0.03 μg/kg to 0.1 μg/kg and 0.11 μg/kg to 0.33 μg/kg for wet weight samples, respectively. Repeatability and reproducibility of the analytical method were achieved below 12.3% and 19.1%, respectively. Recovery ranged from 75.5% to 86.6%. Matrix effect was investigated and presented. The validated analytical method was then applied to analyze 11 pesticides in five Vietnamese cannon eggplant samples. Experimental results indicated that some pesticides were detected in the samples analyzed. However, the concentration of these compounds was below the Vietnam Ministry of Health and EU standards.
Keywords
MS/MS, matrix effect, pesticides, QuEChERS, UPLC
Article Details
References
[2] Vietnam Ministry of Agriculture and Rural Development, Circular No 09/2023/TT-BNNPTNT promulgating lists of approved and prohibited plant protection chemicals in Vietnam pp. 1-283, 2023. [Online]. Available: https://www.ncbi.nlm.nih.gov/books/NBK558907/
[3] Vietnam Minítry of Health, Circular No 50/2016/TTBYT regulations on maximum residue levels of pesticide in food, vol. 14, no. 1, pp. 1-123, 2017.
[4] M. D. H. Prodhan, E. N. Papadakis, and E. Papadopoulou-Mourkidou, Determination of multiple pesticide residues in eggplant with liquid chromatography-mass spectrometry, Food Analytical Methods, vol. 8, no. 1, pp. 229-235, 2015.
https://doi.org/10.1007/s12161-014-9898-3
[5] Y. Zeng, T. Lan, X. Li, Y. Chen, Q. Yang, B. Qu, Y. Zhang, and C. Pan, A comparison of the determination of multiple pesticide residues in fruits, vegetables, and edible fungi using gas chromatography combined with filtration purification and solid-phase extraction, RSC Advances, vol. 14, iss. 24, pp. 16898-16911, 2024.
https://doi.org/10.1039/D3RA07584B
[6] J. F. García-Reyes, B. Gilbert-López, D. Moreno-González, M. Beneito-Cambra, and A. Molina-Díaz, High-resolution mass spectrometry for the analysis of pesticide residues in food, Encyclopedia of Analytical Chemistry, pp. 1-25, Dec. 2018.
https://doi.org/10.1002/9780470027318.a9604
[7] A. Cappiello, P. Palma, G. Famiglini, and V. Termopoli, High-Performance Liquid Chromatography/Mass Spectrometry Methods in Pesticide Analysis. In Encyclopedia of Analytical Chemistry, R.A. Meyers (Ed.), 2017.
https://doi.org/10.1002/9780470027318.a1713.pub2
[8] EU, Commission Implementing Regulation (EU) 2021/808 of 22 March 2021 on the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results, as well as on the methods to, Official Journal of the European Union, vol. 180, no. 401, pp. L180/84-L180/109, 2021. [Online]. Available:
http://extwprlegs1.fao.org/docs/pdf/eur203999.pdf
[9] European Commission, Document SANTE 11312/2021. Analytical quality control and method validation procedures for pesticide residues analysis in food
and feed, pp. 1-57, 2021. [Online]. Available: https://www.eurl-pesticides.eu/docs/public/tmplt_article.asp?CntID=727
[10] ISO 874-1980, ISO 874. Fresh fruits and vegetables -
Sampling Fruits, vol. 1980, pp. 1-13, 1980,
[Online]. Available:
https://standards.iteh.ai/catalog/standards/sist/42b0a7c7
-ea60-47a3-ab61- a3d5f4c01dae/iso-874-1980
[11] N. Vu-Duc, T. Nguyen-Quang, T. Le-Minh, X. Nguyen-
Thi, T. Manh Tran, H. Anh Vu, L. Anh Nguyen, T Doan-
Duy, B. Van Hoi, C. Tu Vu, D. Le-Van, L. A Phung-Thi,
H. A. Vu-Thi, D. Binh Chu, Multiresidue pesticides
analysis of vegetables in Vietnam by ultrahighperformance
liquid chromatography in combination with
high-resolution mass spectrometry (UPLC-Orbitrap
MS), Journal of Analytical Methods in Chemistry,
vol. 2019, iss. 1, pp. 1-12, May 2019.
https://doi.org/10.1155/2019/3489634
[12] P. Khatun, A. Islam, S. Sachi, M. Z. Islam, and P. Islam,
Pesticides in vegetable production in Bangladesh: A
systemic review of contamination levels and associated
health risks in the last decade, Toxicology Reports,
vol. 11, pp. 199-211, Dec. 2023.
https://doi.org/10.1016/j.toxrep.2023.09.003
[13] AOAC International, Appendix F: Guidelines for
Standard Method Performance Requirements, AOAC
Official Methods of Analysis, p. 9, 2016.
[14] K. Weitzel, F. Chemie, M. S. Rev, I. Introduction, and
C. Reference, An overview of matrix effects in liquid
chromatography-mass spectrometry, Mass Spectrometry
Reviews, no. i, pp. 221-235, 2011.
[15] B. K. Matuszewski, M. L. Constanzer, and C. M.
Chavez-Eng, Strategies for the assessment of matrix
effect in quantitative bioanalytical methods based on
HPLC - MS/MS, Analytical Chemistry, vol. 75,
iss. 13, pp. 3019-3030, Jun. 2003.
https://doi.org/10.1021/ac020361s
[16] Helga Trufelli, Pierangela Palma, Giorgio Famiglini, and
A. Cappiello, An overview of matrix effects in liquid
chromatography-mass spectrometry, Mass Spectrometry
Reviews, vol. 30, iss 3, pp. 491-509, Oct. 2010.
https://doi.org/10.1002/mas.20298
[17] Commission regulation, regulation (EC) No 396/2005
of the European Parliament of the Council, vol. 1881,
no. February 1998, pp. 1-5, Dec. 2006,
[Online]. Available:
https://eurlex.europa.eu/LexUriServ/LexUriServ.do?
uri=CONSLEG:2006R1881:20100701:EN:PDF