The influence of the kinds of nitrogen và the light in Ganoderma lucidum submerged culture
Main Article Content
Abstract
The survey was conducted to select the nitrogen sources and suitable light conditions during Viet Nam G. lucidum submerged culture (strain DL). The results showed that organic nitrogen is more suitable than inorganic nitrogen for the growth and synthesis of fungal polysaccharides. The amounts of IPS and EPS in this strain was highest when combined 7.5 g/l yeast and 2.5 g/l peptone. The light affected on the size, shape and the color of the DL cells in submerged culture. Under optimum conditions for nitrogen and light sources, the biomass, IPS and EPS content of DL strain were 13.897 ± 0.274 g DW/l, 15.33 ± 0.97 mg/100mg DW and 2349.76 ± 46.94 mg/l, respectively.
Keywords
Ganoderma lucidum, light, nitrogen, polysaccharides, submerged culture
Article Details
References
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[10] Nguyễn Phan Khánh Hòa, Lê Thị Thủy Tiên, Nguyễn Đức Lượng, Khảo sát sự ảnh hưởng của loại và nồng độ đường lên sự gia tăng sinh khối và sinh tổng hợp polysaccharide trong nuôi cấy chìm Ganoderma lucidum, Tạp chí Khoa học và Công nghệ, 125 (2018) 95-101.
[11] Dubois M., Gilles K. A., Hamilton J. K. , Rebers P. A., Smith F., Colorimetric method for determination of sugars and related substances, Analytical Chemistry 28 (1956) 350 - 356. https://doi.org/10.1021/ac60111a017
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[13] Nguyễn Đức Lượng, Vi sinh học công nghiệp - Tập 2, Nhà xuất bản Đại học Quốc Gia TP Hồ Chí Minh, 2003.
[2] Hikino H., Konno C., Mirin Y., Hayashi T., Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies, Planta Medica, 4 (1985) 339 - 34028. https://doi.org/10.1055/s-2007-969507
[3] Miyazaki Toshio, Nishijima Motogiro, Studies on fungal polysaccharides XXVII: Structural examination of a water-soluble, antitumor polysaccharides of Ganoderma lucidum, Chemical and Pharmaceutical Bulletin, 29 (1981) 3611 - 3616. https://doi.org/10.1248/cpb.29.3611
[4] Sone Y., Okuda R., Wada N., Kishida E., Misaki A., Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing fermentation of mycelium of Ganoderma lucidum, Agricultural and Biological Chemistry, 49 (1985) 2641 - 2653, 45. https://doi.org/10.1080/00021369.1985.10867134
[5] Georges M. Halpern, Healing mushrooms, 188, Square One, United States of America, 2007.
[6] S. Jong, J. M. Birmingham, Medicinal benefits of the mushroom Ganoderma, Advances in Applied Microbiology, 37 (1992) 101 - 134. https://doi.org/10.1016/S0065-2164(08)70253-3
[7] Mizuno T., The extraction and development of antitumor-active polysaccharides from medicinal mushrooms in Japan, International Journal of Medicinal Mushroom, 1 (1999) 9 - 29. https://doi.org/10.1615/IntJMedMushrooms.v1.i1.20
[8] Fang Qing Hua, Zhong Jian Jiang, Submerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites - ganoderic acid and polysaccharides, Biochemical Engineering Journal, 10 (2002) 61-65. https://doi.org/10.1016/S1369-703X(01)00158-9
[9] Yang F. C., Haung H. C., Yang M. J., The influence of environmental conditions on the mycelial growth of Antrodia cinnamomea in submerged cultures, Enzyme and Microbial Technology, 33 (2003) 395-402. https://doi.org/10.1016/S0141-0229(03)00136-4
[10] Nguyễn Phan Khánh Hòa, Lê Thị Thủy Tiên, Nguyễn Đức Lượng, Khảo sát sự ảnh hưởng của loại và nồng độ đường lên sự gia tăng sinh khối và sinh tổng hợp polysaccharide trong nuôi cấy chìm Ganoderma lucidum, Tạp chí Khoa học và Công nghệ, 125 (2018) 95-101.
[11] Dubois M., Gilles K. A., Hamilton J. K. , Rebers P. A., Smith F., Colorimetric method for determination of sugars and related substances, Analytical Chemistry 28 (1956) 350 - 356. https://doi.org/10.1021/ac60111a017
[12] Lê Xuân Thám, Nấm linh chi - dược liệu quý ở Việt Nam, Nhà xuất bản Mũi Cà Mau, 1996.
[13] Nguyễn Đức Lượng, Vi sinh học công nghiệp - Tập 2, Nhà xuất bản Đại học Quốc Gia TP Hồ Chí Minh, 2003.