Development of electrochemical DNA biosensors based highly ordered gold nanoparticle arrays
Main Article Content
Abstract
The analysis of deoxyribonucleic acid (DNA) plays a crucial role in the diagnosis of genetic and DNA-related diseases such as cancer, anaemia, and cystic fibrosis. Conventional techniques, including polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE), provide high analytical accuracy but are often time-consuming, labour-intensive, and costly. Therefore, there is a need for simpler and more cost-effective detection strategies. In this study, we present a nanostructured electrochemical platform for DNA detection based on a highly ordered gold nanoparticle (AuNP) array. The sensing mechanism relies on electrochemical impedance spectroscopy (EIS) to monitor DNA structural changes in real time without labelling. Chemically induced denaturation of double-stranded DNA was investigated to evaluate the sensing performance. A significant impedance variation of approximately 20% was observed during the denaturation process, demonstrating the sensitivity of the AuNP-based platform to DNA conformational changes. These results confirm the feasibility of using ordered AuNP arrays combined with EIS as a simple, label-free, and cost-effective approach for monitoring DNA interactions, offering promising potential for biomedical diagnostic applications.
Keywords
DNA biosensors, gold nanoparticles, EIS biosensors, block copolymers, ordered nanoparticles.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
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